Difference between revisions of "NadE"

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Revision as of 12:30, 16 May 2013

  • Description: NH3-dependent NAD+ synthetase

Gene name nadE
Synonyms outB, tscBGH, gsp-81
Essential yes PubMed
Product NH3-dependent NAD+ synthetase
Function NAD biosynthesis
Gene expression levels in SubtiExpress: nadE
MW, pI 30 kDa, 4.888
Gene length, protein length 816 bp, 272 aa
Immediate neighbours ycgI, tmrB
Sequences Protein DNA DNA_with_flanks
Genetic context
NadE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NadE expression.png















Categories containing this gene/protein

biosynthesis of cofactors, general stress proteins (controlled by SigB), essential genes

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU03130

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+ (according to Swiss-Prot)
  • Protein family: NAD synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced by stress (SigB) PubMed
    • induced by antibiotics interacting with the cytoplasmic membrane PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References