Difference between revisions of "Ldh"
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Revision as of 12:30, 16 May 2013
- Description: L-lactate dehydrogenase
| Gene name | ldh |
| Synonyms | lctE |
| Essential | no |
| Product | L-lactate dehydrogenase |
| Function | overflow metabolism, fermentation |
| Gene expression levels in SubtiExpress: ldh | |
| Interactions involving this protein in SubtInteract: Ldh | |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 34 kDa, 5.394 |
| Gene length, protein length | 963 bp, 321 aa |
| Immediate neighbours | amyE, lctP |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU03050
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-lactate + NAD+ = pyruvate + NADH (according to Swiss-Prot)
- Protein family: LDH family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylation on Tyr-224 PubMed
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- forms tetramers PubMed
- Localization:
- cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P13714
- KEGG entry: [3]
- E.C. number: 1.1.1.27
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional references: PubMed
Shanshan Li, Di Huang, Yong Li, Jianping Wen, Xiaoqiang Jia
Rational improvement of the engineered isobutanol-producing Bacillus subtilis by elementary mode analysis.
Microb Cell Fact: 2012, 11;101
[PubMed:22862776]
[WorldCat.org]
[DOI]
(I e)
Susana Romero, Enrique Merino, Francisco Bolívar, Guillermo Gosset, Alfredo Martinez
Metabolic engineering of Bacillus subtilis for ethanol production: lactate dehydrogenase plays a key role in fermentative metabolism.
Appl Environ Microbiol: 2007, 73(16);5190-8
[PubMed:17586670]
[WorldCat.org]
[DOI]
(P p)
Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307]
[WorldCat.org]
[DOI]
(P p)
Jonas T Larsson, Annika Rogstam, Claes von Wachenfeldt
Coordinated patterns of cytochrome bd and lactate dehydrogenase expression in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 10);3323-3335
[PubMed:16207915]
[WorldCat.org]
[DOI]
(P p)
