PurT

• Description: phosphoribosylglycinamide formyltransferase 2
  
  

Gene name	purT
Synonyms
Essential	no
Product	phosphoribosylglycinamide formyltransferase 2
Function	purine biosynthesis
Gene expression levels in SubtiExpress: purT
Metabolic function and regulation of this protein in SubtiPathways: Purine synthesis, Nucleotides (regulation)
MW, pI	41 kDa, 4.722
Gene length, protein length	1152 bp, 384 aa
Immediate neighbours	ybfI, mpr
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

The gene

Basic information

• Locus tag: BSU02230

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + diphosphate (according to Swiss-Prot)

• Protein family: ATP-grasp domain (according to Swiss-Prot)

• Paralogous protein(s): PurK

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1KJ9 (with Mg-ATP from Escherichia coli, 55% identity, 69% similarity) PubMed

• UniProt: P39771

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: purT (according to DBTBS)

• Expression browser: purT PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

H H Saxild, J H Jacobsen, P Nygaard
Functional analysis of the Bacillus subtilis purT gene encoding formate-dependent glycinamide ribonucleotide transformylase.
Microbiology (Reading): 1995, 141 ( Pt 9);2211-8
[PubMed:7496533] [WorldCat.org] [DOI] (P p)