Difference between revisions of "AraA"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[araB]]'', ''[[abnA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[araB]]'', ''[[abnA]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28800 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28800 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28800 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28800 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28800 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28800 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:araA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:araA_context.gif]]

Revision as of 10:57, 14 May 2013

  • Description: L-arabinose isomerase

Gene name araA
Synonyms
Essential no
Product L-arabinose isomerase
Function arabinose utilization
Gene expression levels in SubtiExpress: araA
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 56 kDa, 5.474
Gene length, protein length 1494 bp, 498 aa
Immediate neighbours araB, abnA
Sequences Protein DNA DNA_with_flanks
Genetic context
AraA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AraA expression.png




























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AraR regulon, CcpA regulon

The gene

Basic information

  • Locus tag: BSU28800

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-arabinose = L-ribulose (according to Swiss-Prot)
  • Protein family: arabinose isomerase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: k (cat) of 14,504 min(-1) and k (cat)/K (m) of 121 min(-1) mM(-1) for L-arabinose PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2AJT (from Escherichia coli, 51% identity, 71% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jin-Ha Kim, Ponnandy Prabhu, Marimuthu Jeya, Manish Kumar Tiwari, Hee-Jung Moon, Raushan Kumar Singh, Jung-Kul Lee
Characterization of an L-arabinose isomerase from Bacillus subtilis.
Appl Microbiol Biotechnol: 2010, 85(6);1839-47
[PubMed:19727704] [WorldCat.org] [DOI] (I p)

Irina Saraiva Franco, Luís Jaime Mota, Cláudio Manuel Soares, Isabel de Sá-Nogueira
Functional domains of the Bacillus subtilis transcription factor AraR and identification of amino acids important for nucleoprotein complex assembly and effector binding.
J Bacteriol: 2006, 188(8);3024-36
[PubMed:16585763] [WorldCat.org] [DOI] (P p)

José Manuel Inácio, Carla Costa, Isabel de Sá-Nogueira
Distinct molecular mechanisms involved in carbon catabolite repression of the arabinose regulon in Bacillus subtilis.
Microbiology (Reading): 2003, 149(Pt 9);2345-2355
[PubMed:12949161] [WorldCat.org] [DOI] (P p)

L J Mota, L M Sarmento, I de Sá-Nogueira
Control of the arabinose regulon in Bacillus subtilis by AraR in vivo: crucial roles of operators, cooperativity, and DNA looping.
J Bacteriol: 2001, 183(14);4190-201
[PubMed:11418559] [WorldCat.org] [DOI] (P p)

L J Mota, P Tavares, I Sá-Nogueira
Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis.
Mol Microbiol: 1999, 33(3);476-89
[PubMed:10417639] [WorldCat.org] [DOI] (P p)

Isabel Sa-Nogueira, Teresa V Nogueira, Snia Soares, Hermnia de Lencastre
The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression.
Microbiology (Reading): 1997, 143 ( Pt 3);957-969
[PubMed:9084180] [WorldCat.org] [DOI] (P p)

I Sá-Nogueira, H de Lencastre
Cloning and characterization of araA, araB, and araD, the structural genes for L-arabinose utilization in Bacillus subtilis.
J Bacteriol: 1989, 171(7);4088-91
[PubMed:2500424] [WorldCat.org] [DOI] (P p)