Difference between revisions of "PbpG"

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|style="background:#ABCDEF;" align="center"|'''Function''' || bifunctional glucosyltransferase/ transpeptidase
 
|style="background:#ABCDEF;" align="center"|'''Function''' || bifunctional glucosyltransferase/ transpeptidase
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU37510 pbpG]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37510 pbpG]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 71 kDa, 9.392   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 71 kDa, 9.392   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[speE]]'', ''[[ywhD]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[speE]]'', ''[[ywhD]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15778&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37510 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37510 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37510 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ywhE_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ywhE_context.gif]]

Revision as of 14:07, 13 May 2013

Gene name pbpG
Synonyms ywhE
Essential no
Product penicillin-binding protein 2d
Function bifunctional glucosyltransferase/ transpeptidase
Gene expression levels in SubtiExpress: pbpG
MW, pI 71 kDa, 9.392
Gene length, protein length 1941 bp, 647 aa
Immediate neighbours speE, ywhD
Sequences Protein DNA Advanced_DNA
Genetic context
YwhE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PbpG expression.png
























Categories containing this gene/protein

cell wall synthesis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigF regulon, SigG regulon

The gene

Basic information

  • Locus tag: BSU37510

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: pbpG (according to DBTBS)
  • Regulation:
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

D C McPherson, A Driks, D L Popham
Two class A high-molecular-weight penicillin-binding proteins of Bacillus subtilis play redundant roles in sporulation.
J Bacteriol: 2001, 183(20);6046-53
[PubMed:11567005] [WorldCat.org] [DOI] (P p)

L B Pedersen, K Ragkousi, T J Cammett, E Melly, A Sekowska, E Schopick, T Murray, P Setlow
Characterization of ywhE, which encodes a putative high-molecular-weight class A penicillin-binding protein in Bacillus subtilis.
Gene: 2000, 246(1-2);187-96
[PubMed:10767540] [WorldCat.org] [DOI] (P p)