Difference between revisions of "MaeA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || malate utilization   
 
|style="background:#ABCDEF;" align="center"|'''Function''' || malate utilization   
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU37050 maeA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37050 maeA]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywkB]]'', ''[[tdk]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywkB]]'', ''[[tdk]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15722&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37050 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ywkA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ywkA_context.gif]]

Revision as of 14:05, 13 May 2013

  • Description: malic enzyme

Gene name maeA
Synonyms ywkA
Essential no
Product NAD-dependent malate dehydrogenase
Function malate utilization
Gene expression levels in SubtiExpress: maeA
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 63 kDa, 4.883
Gene length, protein length 1746 bp, 582 aa
Immediate neighbours ywkB, tdk
Sequences Protein DNA Advanced_DNA
Genetic context
YwkA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MaeA expression.png















Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

MalR regulon

The gene

Basic information

  • Locus tag: BSU37050

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate --> pyruvate
  • Protein family: malic enzymes family (according to Swiss-Prot)
  • Paralogous protein(s): MalS

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the presence of malate (MalR) PubMed
  • Regulatory mechanism:
  • Additional information:
    • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

  • Mutant: GP1141 (aphA3) available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Your additional remarks

References