maeA

168

malic enzyme, required for malate dependent catabolite repression of the pftA-pftB operon

Locus: BSU_37050

Molecular weight: 63.93 kDa

Isoelectric point: 4.88

Protein length: 582 aa

Gene length: 1749 bp

Function: malate utilization

Product: NAD-dependent malate dehydrogenase

Essential: no

E.C.: 1.1.1.38

Synonyms: maeA, ywkA

Genomic Context

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List of homologs in different organisms, belongs to COG0281 (Galperin et al., 2021)

Gene

Coordinates: 3,800,418  3,802,166

The protein

Catalyzed reaction/ biological activity

• (S)-malate + NAD⁺ --> CO₂ + NADH + pyruvate (according to UniProt)
• H⁺ + oxaloacetate --> CO₂ + pyruvate (according to UniProt)

Protein family

• malic enzymes family (according to UniProt)

Cofactors

• NAD⁺

Structure

• 1LLQ (PDB) (from Ascaris suum, 41% identity) PubMed
• P45868 (AlphaFold)

Paralogous protein(s)

• MalS

Expression and Regulation

Operons

• Genes: maeA-ywkB

  Description: PubMed

  Regulation

  • expressed in the presence of malate (MalR) PubMed

  Regulatory mechanism

  • MalR: activation, PubMed, in malR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• MGNA-B657 (ywkA::erm), available at the NBRP B. subtilis, Japan
• GP1141 (aphA3) available in Jörg Stülke's lab
• BKE37050 (maeA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTAAAATATGACCGATC,  downstream forward: _UP4_TAATTAGCGAGTAAATTTTT
• BKK37050 (maeA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTAAAATATGACCGATC,  downstream forward: _UP4_TAATTAGCGAGTAAATTTTT

Labs working on this gene/protein

• Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

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