Difference between revisions of "Obg"

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of the [[phosphorelay]] that activates [[Spo0A]]
 
of the [[phosphorelay]] that activates [[Spo0A]]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU27920 obg]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27920 obg]
 
of the [[phosphorelay]] that activates [[Spo0A]]
 
of the [[phosphorelay]] that activates [[Spo0A]]
 
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|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||'' [[pheB]]'', ''[[spo0B]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||'' [[pheB]]'', ''[[spo0B]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14752&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27920 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27920 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27920 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:obg_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:obg_context.gif]]

Revision as of 13:28, 13 May 2013

  • Description: GTP-binding protein involved in initiation of sporulation and assembly of the 50S ribosome subunit

Gene name obg
Synonyms
Essential Yes
Product GTP-binding protein
Function ribosome assembly, may be required to stimulate activity

of the phosphorelay that activates Spo0A

Gene expression levels in SubtiExpress: obg

of the phosphorelay that activates Spo0A

Interactions involving this protein in SubtInteract: Obg
MW, pI 47 kDa, 4.859
Gene length, protein length 1284 bp, 428 aa
Immediate neighbours pheB, spo0B
Sequences Protein DNA Advanced_DNA
Genetic context
Obg context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Obg expression.png
























Categories containing this gene/protein

translation, phosphorelay, essential genes, GTP-binding proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU27920

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: GTP1/OBG family (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:

Obg possesses 3 domains: A N-terminal glycine-rich domain (1–158), a GTP binding domain (159–342) and a C-terminal TGS domain (343–428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.

  • Modification:
  • Cofactor(s):

GDP, GTP and probably ppGpp

  • Effectors of protein activity:

Interaction with Rsb proteins.

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in B. subtilis.

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP835, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP843, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Natalie Verstraeten, Maarten Fauvart, Wim Versées, Jan Michiels
The universally conserved prokaryotic GTPases.
Microbiol Mol Biol Rev: 2011, 75(3);507-42, second and third pages of table of contents
[PubMed:21885683] [WorldCat.org] [DOI] (I p)

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)

Original publications