Difference between revisions of "RecO"

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|style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA repair/ recombination]]
 
|style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA repair/ recombination]]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU25280 recO]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25280 recO]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RecO RecO]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RecO RecO]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glyQ]]'', ''[[yqzL]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glyQ]]'', ''[[yqzL]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14457&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25280 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25280 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25280 Advanced_DNA]
 
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Revision as of 13:20, 13 May 2013

  • Description: mediator of RecA binding to ssDNA

Gene name recO
Synonyms yqxN, yqfI
Essential no
Product mediator of RecA binding to ssDNA
Function DNA repair/ recombination
Gene expression levels in SubtiExpress: recO
Interactions involving this protein in SubtInteract: RecO
MW, pI 29 kDa, 8.208
Gene length, protein length 765 bp, 255 aa
Immediate neighbours glyQ, yqzL
Sequences Protein DNA Advanced_DNA
Genetic context
RecO context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RecO expression.png
























Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU25280

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • provides RecA access to ssDNA during chromosomal transformation (together with DprA) PubMed
    • catalyzes annealing of SsbA or SsbA/SsbB coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation PubMed
  • Protein family: recO family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • cytoplasm (according to Swiss-Prot)
    • localizes to the DNA entry pole during transformation PubMed

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Tribhuwan Yadav, Begoña Carrasco, Angela R Myers, Nicholas P George, James L Keck, Juan C Alonso
Genetic recombination in Bacillus subtilis: a division of labor between two single-strand DNA-binding proteins.
Nucleic Acids Res: 2012, 40(12);5546-59
[PubMed:22373918] [WorldCat.org] [DOI] (I p)

Candela Manfredi, Yuki Suzuki, Tribhuwan Yadav, Kunio Takeyasu, Juan C Alonso
RecO-mediated DNA homology search and annealing is facilitated by SsbA.
Nucleic Acids Res: 2010, 38(20);6920-9
[PubMed:20581116] [WorldCat.org] [DOI] (I p)

Dawit Kidane, Begoña Carrasco, Candela Manfredi, Katharina Rothmaier, Silvia Ayora, Serkalem Tadesse, Juan C Alonso, Peter L Graumann
Evidence for different pathways during horizontal gene transfer in competent Bacillus subtilis cells.
PLoS Genet: 2009, 5(9);e1000630
[PubMed:19730681] [WorldCat.org] [DOI] (I p)

Candela Manfredi, Begoña Carrasco, Silvia Ayora, Juan C Alonso
Bacillus subtilis RecO nucleates RecA onto SsbA-coated single-stranded DNA.
J Biol Chem: 2008, 283(36);24837-47
[PubMed:18599486] [WorldCat.org] [DOI] (P p)

Joanna Timmins, Ingar Leiros, Sean McSweeney
Crystal structure and mutational study of RecOR provide insight into its mode of DNA binding.
EMBO J: 2007, 26(13);3260-71
[PubMed:17581636] [WorldCat.org] [DOI] (P p)