Difference between revisions of "MtlD"

From SubtiWiki
Jump to: navigation, search
Line 14: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || mannitol utilization  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || mannitol utilization  
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU03990 mtlD]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03990 mtlD]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
Line 24: Line 24:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mtlF]]'', ''[[ycsA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mtlF]]'', ''[[ycsA]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12207&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03990 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03990 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03990 Advanced_DNA]
 
|-
 
|-
 
|-
 
|-

Revision as of 12:09, 13 May 2013

  • Description: mannitol-1-phosphate 5-dehydrogenase

Gene name mtlD
Synonyms mtlB
Essential no
Product mannitol-1-phosphate 5-dehydrogenase
Function mannitol utilization
Gene expression levels in SubtiExpress: mtlD
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 40 kDa, 6.291
Gene length, protein length 1098 bp, 366 aa
Immediate neighbours mtlF, ycsA
Sequences Protein DNA Advanced_DNA
Genetic context
MtlD context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

MtlR regulon

The gene

Basic information

  • Locus tag: BSU03990

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH (according to Swiss-Prot)
  • Protein family: mannitol dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3H2Z (from Shigella flexneri 2a str. 2457t mutant, 42% identity, 59% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Philippe Joyet, Meriem Derkaoui, Sandrine Poncet, Josef Deutscher
Control of Bacillus subtilis mtl operon expression by complex phosphorylation-dependent regulation of the transcriptional activator MtlR.
Mol Microbiol: 2010, 76(5);1279-94
[PubMed:20444094] [WorldCat.org] [DOI] (I p)

Shouji Watanabe, Miyuki Hamano, Hiroshi Kakeshita, Keigo Bunai, Shigeo Tojo, Hirotake Yamaguchi, Yasutaro Fujita, Sui-Lam Wong, Kunio Yamane
Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and glucitol assimilation in Bacillus subtilis: possible cooperation of mtl and gut operons.
J Bacteriol: 2003, 185(16);4816-24
[PubMed:12897001] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)