Difference between revisions of "GlsA"
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|style="background:#ABCDEF;" align="center"|'''Function''' || glutamine degradation | |style="background:#ABCDEF;" align="center"|'''Function''' || glutamine degradation | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU02430 glsA] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glnT]]'', ''[[glnK]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glnT]]'', ''[[glnK]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU02430 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU02430 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU02430 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:ybgJ_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ybgJ_context.gif]] | ||
Revision as of 12:02, 13 May 2013
- Description: glutaminase, low affinity for glutamine
| Gene name | glsA |
| Synonyms | ybgJ |
| Essential | no |
| Product | glutaminase |
| Function | glutamine degradation |
| Gene expression levels in SubtiExpress: glsA | |
| Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate | |
| MW, pI | 36 kDa, 5.315 |
| Gene length, protein length | 981 bp, 327 aa |
| Immediate neighbours | glnT, glnK |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU02430
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: l-glutamine + H2O = L-glutamate + NH3 (according to Swiss-Prot)
- Protein family: glutaminase family (according to Swiss-Prot)
- Paralogous protein(s): YlaM
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: O31465
- KEGG entry: [3]
- E.C. number: 3.5.1.2
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Greg Brown, Alex Singer, Michael Proudfoot, Tatiana Skarina, Youngchang Kim, Changsoo Chang, Irina Dementieva, Ekaterina Kuznetsova, Claudio F Gonzalez, Andrzej Joachimiak, Alexei Savchenko, Alexander F Yakunin
Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis.
Biochemistry: 2008, 47(21);5724-35
[PubMed:18459799]
[WorldCat.org]
[DOI]
(I p)
Takenori Satomura, Daisuke Shimura, Kei Asai, Yoshito Sadaie, Kazutake Hirooka, Yasutaro Fujita
Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.
J Bacteriol: 2005, 187(14);4813-21
[PubMed:15995196]
[WorldCat.org]
[DOI]
(P p)
