Difference between revisions of "CysK"

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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of cysteine, control of [[CymR]] activity
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of cysteine, control of [[CymR]] activity
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00730 cysK]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00730 cysK]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CysK CysK]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CysK CysK]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yacD]]'', ''[[pabB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yacD]]'', ''[[pabB]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11849&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00730 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00730 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00730 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:cysK_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:cysK_context.gif]]

Revision as of 11:56, 13 May 2013

Gene name cysK
Synonyms
Essential no
Product trigger enzyme: cysteine synthetase A
Function biosynthesis of cysteine, control of CymR activity
Gene expression levels in SubtiExpress: cysK
Interactions involving this protein in SubtInteract: CysK
Metabolic function and regulation of this protein in SubtiPathways:
Cys, Met & Sulfate assimilation
MW, pI 32 kDa, 5.492
Gene length, protein length 924 bp, 308 aa
Immediate neighbours yacD, pabB
Sequences Protein DNA Advanced_DNA
Genetic context
CysK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CysK expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, transcription factors and their control, trigger enzyme

This gene is a member of the following regulons

CymR regulon, Spx regulon

The gene

Basic information

  • Locus tag: BSU00730

Phenotypes of a mutant

constitutive expression of the cysH-cysP-sat-cysC-ylnD-ylnE-ylnF operon, auxotrophic for cysteine

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: O(3)-acetyl-L-serine + H2S = L-cysteine + acetate (according to Swiss-Prot)
  • Protein family: cysteine synthase/cystathionine beta-synthase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
    • CymR-CysK, the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis PubMed

Database entries

  • Structure: 1Y7L (from Haemophilus influenzae, 39% identity, 52% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed in the presence of cysteine (CymR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

Your additional remarks

References