Difference between revisions of "IspE"
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|style="background:#ABCDEF;" align="center"|'''Function''' || isoprenoid biosynthesis | |style="background:#ABCDEF;" align="center"|'''Function''' || isoprenoid biosynthesis | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00460 ispE] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 6.349 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 6.349 | ||
| Line 22: | Line 22: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sspF]]'', ''[[purR]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sspF]]'', ''[[purR]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00460 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00460 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00460 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:ispE_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ispE_context.gif]] | ||
Revision as of 11:55, 13 May 2013
- Description: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
| Gene name | ispE |
| Synonyms | yabH , ipk |
| Essential | yes PubMed |
| Product | 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase |
| Function | isoprenoid biosynthesis |
| Gene expression levels in SubtiExpress: ispE | |
| MW, pI | 31 kDa, 6.349 |
| Gene length, protein length | 867 bp, 289 aa |
| Immediate neighbours | sspF, purR |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis of lipids, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00460
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (according to Swiss-Prot)
- Protein family: IspE subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P37550
- KEGG entry: [2]
- E.C. number: 2.7.1.148
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547]
[WorldCat.org]
[DOI]
(P p)
