ThdF

• Description: GTP-binding protein, putative tRNA modification GTPase
  
  

Gene name	thdF
Synonyms	mnmE
Essential	no
Product	putative tRNA modification GTPase
Function	tRNA modification
Gene expression levels in SubtiExpress: thdF
Interactions involving this protein in SubtInteract: ThdF
MW, pI	50 kDa, 4.643
Gene length, protein length	1377 bp, 459 aa
Immediate neighbours	gidA, jag
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, GTP-binding proteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU41020

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP

• Protein family: MnmE subfamily (according to Swiss-Prot) Era/Obg family

• Paralogous protein(s): Era, Obg, YphC, YsxC, RbgA, YqeH, ThdF, YyaF, YnbA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GidA-ThdF (suggested by interaction of E. coli counterparts PubMed and by co-localization on the chromosome)

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P25811

• KEGG entry: [3]

• E.C. number:

Additional information

Information on the corresponding protein from E. coli: EcoCyc MnmE required for wild-type 5-methylaminomethyl-2-thiouridine modification of tRNA, interacts with GidA. MnmE also appears to play a role in oxidation of thiophene and furan compounds and regulates glutamate-dependent acid resistance.

Expression and regulation

• Operon: thdF-gidA-rsmG-noc PubMed

• Expression browser: thdF PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

References

Original publications

Silvia Prado, Magda Villarroya, Milagros Medina, M-Eugenia Armengod
The tRNA-modifying function of MnmE is controlled by post-hydrolysis steps of its GTPase cycle.
Nucleic Acids Res: 2013, 41(12);6190-208
[PubMed:23630314] [WorldCat.org] [DOI] (I p)

Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)

Jörg Sievers, Brian Raether, Marta Perego, Jeff Errington
Characterization of the parB-like yyaA gene of Bacillus subtilis.
J Bacteriol: 2002, 184(4);1102-11
[PubMed:11807071] [WorldCat.org] [DOI] (P p)