Difference between revisions of "NtdA"

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* '''Description:''' synthesis of the antibiotic neotrehalosadiamine <br/><br/>
+
* '''Description:''' pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase <br/><br/>
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || sugar aminotransferase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of the antibiotic neotrehalosadiamine
+
|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of the antibiotic kanosamine
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU10550 ntdA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU10550 ntdA]
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
 +
** 3-oxo-d-glucose-6-phosphate + glutamate --> kanosamine-6-phosphate {{PubMed|23586652}}
  
 
* '''Protein family:''' degT/dnrJ/eryC1 family (according to Swiss-Prot)
 
* '''Protein family:''' degT/dnrJ/eryC1 family (according to Swiss-Prot)
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* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
 +
** pyridoxalphosphate {{PubMed|23586652}}
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ntdA_1128286_1129611_-1 ntdA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ntdA_1128286_1129611_-1 ntdA] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigA]] {{PubMed|14612444}}
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|14612444}}
  
 
* '''Regulation:''' induced by 3,3'-neotrehalosadiamine ([[NtdR]]) {{PubMed|14612444}}
 
* '''Regulation:''' induced by 3,3'-neotrehalosadiamine ([[NtdR]]) {{PubMed|14612444}}
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<pubmed> 21512256</pubmed>
 
<pubmed> 21512256</pubmed>
 
==Original publications==
 
==Original publications==
<pubmed>19087206,19342798,17056753 ,14612444  </pubmed>
+
<pubmed>19087206,19342798,17056753 ,14612444  23586652 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 13:37, 19 April 2013

  • Description: pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase

Gene name ntdA
Synonyms yhjL
Essential no
Product pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase
Function synthesis of the antibiotic kanosamine
Gene expression levels in SubtiExpress: ntdA
MW, pI 49 kDa, 5.971
Gene length, protein length 1323 bp, 441 aa
Immediate neighbours ntdB, ntdR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YhjL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NtdA expression.png















Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

NtdR regulon

The gene

Basic information

  • Locus tag: BSU10550

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • 3-oxo-d-glucose-6-phosphate + glutamate --> kanosamine-6-phosphate PubMed
  • Protein family: degT/dnrJ/eryC1 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced by 3,3'-neotrehalosadiamine (NtdR) PubMed
  • Regulatory mechanism: NtdR: positive regulator PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Takashi Inaoka, Kozo Ochi
Activation of dormant secondary metabolism neotrehalosadiamine synthesis by an RNA polymerase mutation in Bacillus subtilis.
Biosci Biotechnol Biochem: 2011, 75(4);618-23
[PubMed:21512256] [WorldCat.org] [DOI] (I p)

Original publications