ntdB

168

kanosamine-6-phosphate phosphatase

Locus: BSU_10540

Molecular weight: 33.05 kDa

Isoelectric point: 5.35

Protein length: 282 aa

Gene length: 849 bp

Function: synthesis of the antibiotic kanosamine

Product: kanosamine-6-phosphate phosphatase

Essential: no

Synonyms: ntdB, yhjK

Genomic Context

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List of homologs in different organisms, belongs to COG0561 (Galperin et al., 2021)

Gene

Coordinates: 1,127,466  1,128,314

The protein

Catalyzed reaction/ biological activity

• kanosamine-6-phosphate --> kanosamine PubMed

Protein family

• HAD superfamily (according to UniProt)
• Cof family (according to UniProt)

Structure

• 3GYG (PDB)
• O07565 (AlphaFold)

Expression and Regulation

Operons

• Genes: ntdA-ntdB-ntdC-glcP

  Description: PubMed

  Regulation

  • induced by 3,3'-neotrehalosadiamine and kanosamine (NtdR) PubMed

  Regulatory mechanism

  • NtdR: activation , in ntdR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A724 (yhjK::erm), available at the NBRP B. subtilis, Japan
• BKE10540 (ntdB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGGATGTTCAACCGTGGATA,  downstream forward: _UP4_ATTGGATCATGAGGAGGAAA
• BKK10540 (ntdB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGGATGTTCAACCGTGGATA,  downstream forward: _UP4_ATTGGATCATGAGGAGGAAA

References

Reviews

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Original Publications

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