CdaA

• Description: maybe involved in the synthesis of c-di-AMP in vegetative cells
  
  

Gene name	ybbP
Synonyms	ybbQ
Essential	no
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: ybbP
Metabolic function and regulation of this protein in SubtiPathways: Murein recycling
MW, pI	30 kDa, 8.074
Gene length, protein length	819 bp, 273 aa
Immediate neighbours	ybbM, ybbR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, metabolism of signalling nucleotides

The gene

Basic information

• Locus tag: BSU01750

Phenotypes of a mutant

• inactivation of ybbP results in severe beta-lactam sensitivity PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): YojJ

Extended information on the protein

• Kinetic information:

• Domains: contains a DAC domain potentially involved in the synthesis of c-di-AMP PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q45589

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ybbP-ybbR-glmM-glmS

• Expression browser: ybbP PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information: the ybbP-ybbR part of the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:
  • GP997 (ybbP::cat), available in Jörg Stülke's lab
  • GP985 (ybbP-ybbR::cat), available in Jörg Stülke's lab

• Expression vector:
  • expression of native ybbP in B. subtilis: pGP1960 (in pBQ200), available in Stülke lab
  • expression of ybbP-Strep in B. subtilis suitable for SPINE: pGP1986 (in pGP382), available in Stülke lab

• lacZ fusion: GP1339 (cat) based on pAC6, available in Jörg Stülke's lab

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmid: pGP1990.

• FLAG-tag construct: GP1331 (ybbP-3xFLAG ermC ganA::(ybbR-Strep aphA3), based on pGP1087), available in Jörg Stülke's lab

• Antibody:

References

Reviews

Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086] [WorldCat.org] [DOI] (I e)

Original publications

Additional publications: PubMed

Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin, Sigal Ben-Yehuda
c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.
EMBO Rep: 2011, 12(6);594-601
[PubMed:21566650] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)