Difference between revisions of "EtfA"

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(Expression and regulation)
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* '''Mutant:'''
 
* '''Mutant:'''
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** 1A856 ( ''etfA''::''cat''), {{PubMed|17085570}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A856&Search=1A856 BGSC]
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 09:04, 19 September 2012

  • Description: electron transfer flavoprotein (alpha subunit)

Gene name etfA
Synonyms
Essential no
Product electron transfer flavoprotein (alpha subunit)
Function fatty acid degradation, calcium carbonate biomineralization
Gene expression levels in SubtiExpress: etfA
Metabolic function and regulation of this protein in SubtiPathways:
Fatty acid degradation
MW, pI 34 kDa, 4.652
Gene length, protein length 975 bp, 325 aa
Immediate neighbours abf2, etfB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
EtfA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
EtfA expression.png




























Categories containing this gene/protein

electron transport/ other, utilization of lipids

This gene is a member of the following regulons

CcpA regulon, FadR regulon

The gene

Basic information

  • Locus tag: BSU28520

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: ETF alpha-subunit/fixB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed

Massimiliano Marvasi, Pieter T Visscher, Brunella Perito, Giorgio Mastromei, Lilliam Casillas-Martínez
Physiological requirements for carbonate precipitation during biofilm development of Bacillus subtilis etfA mutant.
FEMS Microbiol Ecol: 2010, 71(3);341-50
[PubMed:20059546] [WorldCat.org] [DOI] (I p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)

Chiara Barabesi, Alessandro Galizzi, Giorgio Mastromei, Mila Rossi, Elena Tamburini, Brunella Perito
Bacillus subtilis gene cluster involved in calcium carbonate biomineralization.
J Bacteriol: 2007, 189(1);228-35
[PubMed:17085570] [WorldCat.org] [DOI] (P p)