fadB

168

3-hydroxyacyl-CoA dehydratase

Locus: BSU_28540

Molecular weight: 27.40 kDa

Isoelectric point: 4.95

Protein length: 258 aa

Gene length: 777 bp

Function: fatty acid degradation

Product: 3-hydroxyacyl-CoA dehydratase

Essential: no

E.C.: 4.2.1.17

Synonyms: fadB, ysiB

Genomic Context

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List of homologs in different organisms, belongs to COG1024 (Galperin et al., 2021)

Gene

Coordinates: 2,917,166  2,917,942

The protein

Catalyzed reaction/ biological activity

• (3S)-hydroxyacyl-CoA --> (2E)-enoyl-CoA + H₂O (according to UniProt)
• 4-saturated-(3S)-hydroxyacyl-CoA --> (3E)-enoyl-CoA + H₂O (according to UniProt)

Protein family

• enoyl-CoA hydratase/isomerase family (according to UniProt)

Structure

• 3PEA (PDB) (from B. anthracis, 49% identity, 66% similarity)
• P94549 (AlphaFold)

Modification

• phosphorylated on Arg-230 PubMed

Paralogous protein(s)

• YhaR, (34%)

Expression and Regulation

Operons

• Genes: lcfA-fadR-fadB-etfB-etfA

  Description: PubMed

  Regulation

  • induced by long chain acyl-CoA (C14 ... C20) (FadR) PubMed

  Regulatory mechanism

  • FadR: repression, PubMed, in fadR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: fadR-fadB-etfB-etfA

  Description: PubMed

  Regulation

  • induced by long-chain fatty acids (FadR) PubMed

Biological materials

Mutant

• MGNA-B009 (ysiB::erm), available at the NBRP B. subtilis, Japan
• 1A854 ( fadB::cat), PubMed, available at BGSC
• BKE28540 (fadB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCATTCATAGGACAGAACT,  downstream forward: _UP4_GGCGAATAAAAGGGGATATG
• BKK28540 (fadB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCATTCATAGGACAGAACT,  downstream forward: _UP4_GGCGAATAAAAGGGGATATG

References

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