DctP

• Description: uptake of succinate, fumurate, malate and oxaloacetate via proton symport
  
  

Gene name	dctP
Synonyms	ydbH, dctA
Essential	no
Product	C4-dicarboxylate transport protein
Function	uptake of succinate, fumurate, malate and oxaloacetate
Gene expression levels in SubtiExpress: dctP
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	45 kDa, 8.668
Gene length, protein length	1263 bp, 421 aa
Immediate neighbours	dctR, ydbI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

CcpA regulon, FsrA regulon

The gene

Basic information

• Locus tag: BSU04470

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: uptake of succinate, fumurate, malate and oxaloacetate via proton symport PubMed

• Protein family: View classification (according to Swiss-Prot)

• Paralogous protein(s): GltP, GltT

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P96603

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • dctS-dctR-dctP PubMed
  • dctP PubMed

• Expression browser: dctP PubMed

• Sigma factor:
  • dctP: SigA PubMed

• Regulation:
  • repressed by glucose (6.2-fold) (CcpA) PubMed
  • part of the iron sparing response, strong down-regulation in a fur mutant (Fur, FsrA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • Fur: indirect effect via control of fsrA expression PubMed
  • FsrA: translation inhibition PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Gregory T Smaldone, Olga Revelles, Ahmed Gaballa, Uwe Sauer, Haike Antelmann, John D Helmann
A global investigation of the Bacillus subtilis iron-sparing response identifies major changes in metabolism.
J Bacteriol: 2012, 194(10);2594-605
[PubMed:22389480] [WorldCat.org] [DOI] (I p)

Maarten Groeneveld, Ruud G J Detert Oude Weme, Ria H Duurkens, Dirk Jan Slotboom
Biochemical characterization of the C4-dicarboxylate transporter DctA from Bacillus subtilis.
J Bacteriol: 2010, 192(11);2900-7
[PubMed:20363944] [WorldCat.org] [DOI] (I p)

Kousei Tanaka, Kazuo Kobayashi, Naotake Ogasawara
The Bacillus subtilis YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium.
Microbiology (Reading): 2003, 149(Pt 9);2317-2329
[PubMed:12949159] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

Kei Asai, Sang-Hoon Baik, Yasuhiro Kasahara, Shigeki Moriya, Naotake Ogasawara
Regulation of the transport system for C4-dicarboxylic acids in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 2);263-271
[PubMed:10708364] [WorldCat.org] [DOI] (P p)

Ralf Rabus, Donald L Jack, David J Kelly, Milton H Saier
TRAP transporters: an ancient family of extracytoplasmic solute-receptor-dependent secondary active transporters.
Microbiology (Reading): 1999, 145 ( Pt 12);3431-3445
[PubMed:10627041] [WorldCat.org] [DOI] (P p)