BdbD

• Description: thiol-disulfide oxidoreductase, required for the formation of thiol disulfide bonds in several proteins
  
  

Gene name	bdbD
Synonyms	yvgV
Essential	no
Product	thiol-disulfide oxidoreductase
Function	oxidative folding of proteins
Gene expression levels in SubtiExpress: bdbD
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	24 kDa, 5.089
Gene length, protein length	666 bp, 222 aa
Immediate neighbours	bdbC, cadA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, chaperones/ protein folding, sporulation proteins, membrane proteins

This gene is a member of the following regulons

ComK regulon, SigE regulon

The gene

Basic information

• Locus tag: BSU33480

Phenotypes of a mutant

• loss of transformability PubMed
• sensitive to osmotic shock PubMed
• several proteins are absent from the membrane proteome of a bdbC-bdbD mutant: PubMed
  • the membrane proteins BglP, TcyP, SipU, LytA, and YxaI PubMed
  • the cytoplasmic or membrane-associated proteins GlkX, ProA, PyrAA, PyrAB, PyrH, PyrE, PyrF, DegS, YbxA PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • formation of thiol disulfide bonds in ComEC and ComGC (together with BdbC) PubMed

• Protein family: DsbA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Ca (2+) PubMed

• Effectors of protein activity:

• Interactions:

• Localization: membrane, faced to the outer side of the membrane PubMed

Database entries

• Structure: 3EU3 (reduced form), 3EU4 (oxidized form) PubMed

• UniProt: O32218

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: bdbD-bdbC PubMed

• Expression browser: bdbD PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: