Difference between revisions of "YaaO"

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* '''Description:''' similar to arginine decarboxylase, but without enzymatic activity <br/><br/>
+
* '''Description:''' carboxynorspermidine decarboxylase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || putative arginine decarboxylase
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|style="background:#ABCDEF;" align="center"| '''Product''' || carboxynorspermidine decarboxylase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
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|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of norspermidine
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
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<br/><br/><br/><br/>
 
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<br/><br/><br/><br/>
<br/><br/><br/><br/>
 
 
 
 
<br/><br/><br/><br/><br/><br/>
 
<br/><br/><br/><br/><br/><br/>
  
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[poorly characterized/ putative enzymes]]}}
+
{{SubtiWiki category|[[miscellaneous metabolic pathways]]}},
 +
{{SubtiWiki category|[[biofilm formation]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
** impaired biofilm disassembly  {{PubMed|22541437}}
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
 
 
  
  
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
 +
** catalyzes the last step in norspermidine synthesis {{PubMed|22541437}}
  
 
* '''Protein family:''' Orn/Lys/Arg decarboxylase class-I family (according to Swiss-Prot)
 
* '''Protein family:''' Orn/Lys/Arg decarboxylase class-I family (according to Swiss-Prot)
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=References=
 
=References=
<pubmed> 20876533 </pubmed>
+
<pubmed> 20876533 22541437 </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 15:09, 6 May 2012

  • Description: carboxynorspermidine decarboxylase

Gene name yaaO
Synonyms
Essential no
Product carboxynorspermidine decarboxylase
Function synthesis of norspermidine
Metabolic function and regulation of this protein in SubtiPathways:
Nucleotides (regulation)
MW, pI 52 kDa, 5.713
Gene length, protein length 1440 bp, 480 aa
Immediate neighbours yaaN, tmk
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YaaO context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YaaO expression.png























Categories containing this gene/protein

miscellaneous metabolic pathways, biofilm formation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00270

Phenotypes of a mutant

    • impaired biofilm disassembly PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • catalyzes the last step in norspermidine synthesis PubMed
  • Protein family: Orn/Lys/Arg decarboxylase class-I family (according to Swiss-Prot)
  • Paralogous protein(s): SpeA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ilana Kolodkin-Gal, Shugeng Cao, Liraz Chai, Thomas Böttcher, Roberto Kolter, Jon Clardy, Richard Losick
A self-produced trigger for biofilm disassembly that targets exopolysaccharide.
Cell: 2012, 149(3);684-92
[PubMed:22541437] [WorldCat.org] [DOI] (I p)

Matthew Burrell, Colin C Hanfrey, Ewan J Murray, Nicola R Stanley-Wall, Anthony J Michael
Evolution and multiplicity of arginine decarboxylases in polyamine biosynthesis and essential role in Bacillus subtilis biofilm formation.
J Biol Chem: 2010, 285(50);39224-38
[PubMed:20876533] [WorldCat.org] [DOI] (I p)