Difference between revisions of "AbfA"

From SubtiWiki
Jump to: navigation, search
Line 28: Line 28:
 
|colspan="2" | '''Genetic context''' <br/> [[Image:abfA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:abfA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 +
|-
 +
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=abfA_2938330_2939832_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:abfA_expression.png|500px]]
 
|-
 
|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
  
 
<br/><br/><br/><br/><br/><br/>
 
<br/><br/><br/><br/><br/><br/>

Revision as of 10:38, 20 April 2012

  • Description: alpha-L-arabinofuranosidase

Gene name abfA
Synonyms
Essential no
Product alpha-L-arabinofuranosidase
Function arabinan degradation
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 56 kDa, 5.344
Gene length, protein length 1500 bp, 500 aa
Immediate neighbours cstA, araQ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AbfA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AbfA expression.png




























Categories containing this gene/protein

utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

AraR regulon, CcpA regulon

The gene

Basic information

  • Locus tag: BSU28720

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 51 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1QW8 (complex with Ara-alpha-Xyl, Geobacillus stearothermophilus), 1PZ3 (Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

José Manuel Inácio, Isabel Lopes Correia, Isabel de Sá-Nogueira
Two distinct arabinofuranosidases contribute to arabino-oligosaccharide degradation in Bacillus subtilis.
Microbiology (Reading): 2008, 154(Pt 9);2719-2729
[PubMed:18757805] [WorldCat.org] [DOI] (P p)

Maria Paiva Raposo, José Manuel Inácio, Luís Jaime Mota, Isabel de Sá-Nogueira
Transcriptional regulation of genes encoding arabinan-degrading enzymes in Bacillus subtilis.
J Bacteriol: 2004, 186(5);1287-96
[PubMed:14973026] [WorldCat.org] [DOI] (P p)

José Manuel Inácio, Carla Costa, Isabel de Sá-Nogueira
Distinct molecular mechanisms involved in carbon catabolite repression of the arabinose regulon in Bacillus subtilis.
Microbiology (Reading): 2003, 149(Pt 9);2345-2355
[PubMed:12949161] [WorldCat.org] [DOI] (P p)

L J Mota, P Tavares, I Sá-Nogueira
Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis.
Mol Microbiol: 1999, 33(3);476-89
[PubMed:10417639] [WorldCat.org] [DOI] (P p)

Isabel Sa-Nogueira, Teresa V Nogueira, Snia Soares, Hermnia de Lencastre
The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression.
Microbiology (Reading): 1997, 143 ( Pt 3);957-969
[PubMed:9084180] [WorldCat.org] [DOI] (P p)