PbpG

• Description: penicillin-binding protein 2d
  
  

Gene name	pbpG
Synonyms	ywhE
Essential	no
Product	penicillin-binding protein 2d
Function	bifunctional glucosyltransferase/ transpeptidase
MW, pI	71 kDa, 9.392
Gene length, protein length	1941 bp, 647 aa
Immediate neighbours	speE, ywhD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall synthesis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigF regulon, SigG regulon

The gene

Basic information

• Locus tag: BSU37510

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)
  • prespore Link to a summary

Database entries

• Structure:

• UniProt: P70997

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pbpG (according to DBTBS)

• Expression browser: pbpG PubMed

• Sigma factor: SigF, SigG PubMed

• Regulation:
  • expressed during sporulation in the forespore (SigF, SigG) PubMed

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between pbpG and ywhD PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab

• Antibody:

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

D C McPherson, A Driks, D L Popham
Two class A high-molecular-weight penicillin-binding proteins of Bacillus subtilis play redundant roles in sporulation.
J Bacteriol: 2001, 183(20);6046-53
[PubMed:11567005] [WorldCat.org] [DOI] (P p)

L B Pedersen, K Ragkousi, T J Cammett, E Melly, A Sekowska, E Schopick, T Murray, P Setlow
Characterization of ywhE, which encodes a putative high-molecular-weight class A penicillin-binding protein in Bacillus subtilis.
Gene: 2000, 246(1-2);187-96
[PubMed:10767540] [WorldCat.org] [DOI] (P p)