MmgD

• Description: 2-methylcitrate synthase
  
  

Gene name	mmgD
Synonyms	yqiO, prpC
Essential	no
Product	2-methylcitrate synthase
Function	mother cell metabolism
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	41 kDa, 6.202
Gene length, protein length	1116 bp, 372 aa
Immediate neighbours	mmgE, mmgC
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

sporulation proteins, poorly characterized/ putative enzymes

This gene is a member of the following regulons

CcpA regulon, SigE regulon

The gene

Basic information

• Locus tag: BSU24140

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Propanoyl-CoA + H₂O + oxaloacetate = (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA (according to Swiss-Prot)

• Protein family: citrate synthase family (according to Swiss-Prot)

• Paralogous protein(s): CitA, CitZ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P45858

• KEGG entry: [3]

• E.C. number: 2.3.3.5

Expression and regulation

• Operon: mmgA-mmgB-mmgC-mmgD-mmgE-yqiQ PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed
  • repressed by glucose (CcpA) PubMed
  • expression is reduced in a SigV mutant PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

E M Bryan, B W Beall, C P Moran
A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis.
J Bacteriol: 1996, 178(16);4778-86
[PubMed:8759838] [WorldCat.org] [DOI] (P p)