YrhJ

• Description: cytochrome P450 (CYP102A3)/ NADPH-cytochrome P450 reductase
  
  

Gene name	yrhJ
Synonyms	cypE
Essential	no
Product	NADPH-cytochrome P450 reductase
Function	fatty acid metabolism
MW, pI	118 kDa, 6.017
Gene length, protein length	3162 bp, 1054 aa
Immediate neighbours	yrhK, fatR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

electron transport/ other, lipid metabolism/ other, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

FatR regulon, SigM regulon, SigW regulon, SigX regulon

The gene

Basic information

• Locus tag: BSU27160

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • hydroxylates medium-chain fatty acids in subterminal positions PubMed

• Protein family:

• Paralogous protein(s): YetO

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure: 2X7Y (from Bacillus megaterium; 67% identity, 88% similarity)

• UniProt: O08336

• KEGG entry: [3]

• E.C. number: 1.6.2.4

Expression and regulation

• Operon:
  • fatR-yrhJ PubMed
  • yrhH-fatR-yrhJ PubMed

• Sigma factor: SigM PubMed, SigW PubMed, SigX PubMed

• Regulation:
  • induced by unsaturated fatty acids (FatR) PubMed
  • expression is reduced in a SigV mutant PubMed

• Regulatory mechanism:
  • FatR: transcription repression PubMed

• Additional information:
  • A ncRNA is predicted between yrhK and yrhJ PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed