Med

• Description: lipoprotein, positive regulator of comK, stimulates KinD-dependent phosphorylation of Spo0A
  
  

Gene name	med
Synonyms	yjaW
Essential	no
Product	positive regulator of comK
Function	regulation of competence
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	35 kDa, 4.599
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	yjaV, comZ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

genetic competence

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU11300

Expression

Phenotypes of a mutant

• impaired biofilm formation PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • stimulates KinD-dependent phosphorylation of Spo0A PubMed

• Protein family: BMP lipoprotein family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell surface PubMed

Database entries

• Structure:

• UniProt: O32436

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: med-comZ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additonal publications: PubMed

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hitoshi Hashimoto, Teruo Tanaka
Med, a cell-surface localized protein regulating a competence transcription factor gene, comK, in Bacillus subtilis.
Biosci Biotechnol Biochem: 2002, 66(4);892-6
[PubMed:12036071] [WorldCat.org] [DOI] (P p)

M Ogura, Y Ohshiro, S Hirao, T Tanaka
A new Bacillus subtilis gene, med, encodes a positive regulator of comK.
J Bacteriol: 1997, 179(20);6244-53
[PubMed:9335269] [WorldCat.org] [DOI] (P p)