TrpP

• Description: L-tryptophan transporter (proton symport)
  
  

Gene name	trpP
Synonyms	yhaG
Essential	no
Product	L-tryptophan transporter (proton symport)
Function	tryptophan uptake
Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp
MW, pI	18 kDa, 11.155
Gene length, protein length	516 bp, 172 aa
Immediate neighbours	yhaH, serC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

TRAP regulon

The gene

Basic information

• Locus tag: BSU10010

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: trpP transporter family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O07515

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: trpP PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the absence of tryptophan (MtrB) PubMed

• Regulatory mechanism:
  • MtrB: translation inhibition at an RNA switch, the MtrB binding site overlaps the Shine-DAlgarno sequence and translation initation region, MtrB binds in the presence of tryptophan PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Helen Yakhnin, Hong Zhang, Alexander V Yakhnin, Paul Babitzke
The trp RNA-binding attenuation protein of Bacillus subtilis regulates translation of the tryptophan transport gene trpP (yhaG) by blocking ribosome binding.
J Bacteriol: 2004, 186(2);278-86
[PubMed:14702295] [WorldCat.org] [DOI] (P p)

J P Sarsero, E Merino, C Yanofsky
A Bacillus subtilis gene of previously unknown function, yhaG, is translationally regulated by tryptophan-activated TRAP and appears to be involved in tryptophan transport.
J Bacteriol: 2000, 182(8);2329-31
[PubMed:10735881] [WorldCat.org] [DOI] (P p)