yhaH

168

unknown

Locus: BSU_10000

Molecular weight: 12.93 kDa

Isoelectric point: 8.6

Protein length: 118 aa

Gene length: 357 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yhaH

Genomic Context

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List of homologs in different organisms, belongs to COG4980 (Galperin et al., 2021)

Gene

Coordinates: 1,073,895  1,074,251

The protein

Structure

• O07516 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: yhaH

  Description: PubMed

Biological materials

Mutant

• GP3768 (ΔyhaH::kan) available in Jörg Stülke's lab
• GP3770 (ΔyhaH::kan ΔytxH::cat) available in Jörg Stülke's lab
• MGNA-A667 (yhaH::erm), available at the NBRP B. subtilis, Japan
• BKE10000 (yhaH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACAATCACCCCGTCTT,  downstream forward: _UP4_TAATATCCTATTCAAAAGAA
• BKK10000 (yhaH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACAATCACCCCGTCTT,  downstream forward: _UP4_TAATATCCTATTCAAAAGAA

Expression vectors

• pGP3641: expression of yhaH by pBQ200 in B. subtilis, available in Jörg Stülke's lab
• pGP3648: expression of Strep-yhaH by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
• pGP3649: expression of yhaH-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
• pGP3654 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
• pGP3670 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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