SinI

• Description: antagonist of SinR, drives SlrR from the SlrR(LOW) to the SlrR(HIGH) state
  
  

Gene name	sinI
Synonyms
Essential	no
Product	antagonist of SinR
Function	control of biofilm formation
Interactions involving this protein in SubtInteract: SinI
Regulation of this protein in SubtiPathways: Biofilm
MW, pI	6 kDa, 6.333
Gene length, protein length	171 bp, 57 aa
Immediate neighbours	yqhG, sinR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transcription factors and their control, transition state regulators, biofilm formation

This gene is a member of the following regulons

AbrB regulon, ScoC regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU24600

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): SlrA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SinR-SinI PubMed

• Localization:

Database entries

• Structure: 1B0N (complex SinR-SinI) PubMed

• UniProt: P23308

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sinI-sinR PubMed, sinI-Spo0A PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • activated under conditions that trigger sporulation by (Spo0A) interaction PubMed,PubMed
  • repressed during exponential growth (ScoC) PubMed
  • repressed during logrithmic growth (AbrB) PubMed
  • repressed during vegetative growth (SinR) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation, in a portion of cells PubMed
  • ScoC: transcription repression PubMed
  • AbrB: transcription repression PubMed
  • SinR: transcription repression PubMed

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

• Mutant:
  • GP959 (spc), available in Stülke lab
  • DS91 (spc) NCIB3610 derivate, available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• FLAG-tag construct (C-term): GP935 (kan), available in Stülke lab

• Antibody:

References

Reviews

Patrick Piggot
Epigenetic switching: bacteria hedge bets about staying or moving.
Curr Biol: 2010, 20(11);R480-2
[PubMed:20541494] [WorldCat.org] [DOI] (I p)

Modelling of the SinI/SinR switch

Jennifer S Hallinan, Goksel Misirli, Anil Wipat
Evolutionary computation for the design of a stochastic switch for synthetic genetic circuits.
Annu Int Conf IEEE Eng Med Biol Soc: 2010, 2010;768-74
[PubMed:21095906] [WorldCat.org] [DOI] (P p)

Original publications

Additonal publications: PubMed

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947