Difference between revisions of "Fra"
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * severe growth phenotype associated with a broadly disturbed iron homeostasis {{PubMed|21744456}} | ||
=== Database entries === | === Database entries === | ||
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=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| Line 60: | Line 59: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' transfers iron onto the B. subtilis SUF system ([[SufU]]-[[SufS]]) for iron-sulfur cluster biosynthesis {{PubMed|21744456}} |
* '''Protein family:''' | * '''Protein family:''' | ||
| Line 80: | Line 79: | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
** the protein is active as a dimer {{PubMed|20087498}} | ** the protein is active as a dimer {{PubMed|20087498}} | ||
| − | ** ([[ | + | ** ([[Fra]])2-[[SufU]], requires iron {{PubMed|20087498}} |
* '''Localization:''' | * '''Localization:''' | ||
| Line 110: | Line 109: | ||
=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' | + | * '''Mutant:''' available in [[Mohamed Marahiel]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
| Line 127: | Line 126: | ||
=References= | =References= | ||
| − | <pubmed> 20087498 </pubmed> | + | <pubmed> 20087498 21744456 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:23, 12 July 2011
- Description: frataxin-like protein, required for the biosynthesis of iron-sulfur clusters
| Gene name | fra |
| Synonyms | ydhG |
| Essential | no |
| Product | frataxin-like protein |
| Function | intracellular iron channeling, biosynthesis of iron-sulfur clusters |
| MW, pI | 14 kDa, 5.377 |
| Gene length, protein length | 369 bp, 123 aa |
| Immediate neighbours | phoB, ydhH |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU05750
Phenotypes of a mutant
- severe growth phenotype associated with a broadly disturbed iron homeostasis PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transfers iron onto the B. subtilis SUF system (SufU-SufS) for iron-sulfur cluster biosynthesis PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): Fe(2+) is bound with high affinity PubMed
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 2OC6
- UniProt: Q797E6
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: available in Mohamed Marahiel's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Alexander G Albrecht, Hannes Landmann, David Nette, Olaf Burghaus, Florian Peuckert, Andreas Seubert, Marcus Miethke, Mohamed A Marahiel
The frataxin homologue Fra plays a key role in intracellular iron channeling in Bacillus subtilis.
Chembiochem: 2011, 12(13);2052-61
[PubMed:21744456]
[WorldCat.org]
[DOI]
(I p)
Wenbin Qi, J A Cowan
A structural and functional homolog supports a general role for frataxin in cellular iron chemistry.
Chem Commun (Camb): 2010, 46(5);719-21
[PubMed:20087498]
[WorldCat.org]
[DOI]
(I p)
