Difference between revisions of "CotC"
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| + | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[sporulation proteins]]}} | ||
| + | |||
| + | = This gene is a member of the following [[regulons]] = | ||
| + | {{SubtiWiki regulon|[[GerE regulon]]}}, | ||
| + | {{SubtiWiki regulon|[[SigK regulon]]}}, | ||
| + | {{SubtiWiki regulon|[[SpoIIID regulon]]}} | ||
=The gene= | =The gene= | ||
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=The protein= | =The protein= | ||
Revision as of 20:01, 8 December 2010
- Description: spore coat protein (outer)
| Gene name | cotC |
| Synonyms | |
| Essential | no |
| Product | spore coat protein (outer) |
| Function | resistance of the spore |
| MW, pI | 14 kDa, 9.558 |
| Gene length, protein length | 354 bp, 118 aa |
| Immediate neighbours | yncM, tatAC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
GerE regulon, SigK regulon, SpoIIID regulon
The gene
Basic information
- Locus tag: BSU17700
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: outer spore coat, more abundant at the mother cell-distal pole of the forespore PubMed, the CotC-CotU complex assembles around the forming spore PubMed
Database entries
- Structure:
- UniProt: P07790
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: cotC (according to DBTBS)
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Rachele Isticato, Assunta Pelosi, Maurilio De Felice, Ezio Ricca
CotE binds to CotC and CotU and mediates their interaction during spore coat formation in Bacillus subtilis.
J Bacteriol: 2010, 192(4);949-54
[PubMed:20023017]
[WorldCat.org]
[DOI]
(I p)
Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Localization of proteins to different layers and regions of Bacillus subtilis spore coats.
J Bacteriol: 2010, 192(2);518-24
[PubMed:19933362]
[WorldCat.org]
[DOI]
(I p)
Rachele Isticato, Assunta Pelosi, Rita Zilhão, Loredana Baccigalupi, Adriano O Henriques, Maurilio De Felice, Ezio Ricca
CotC-CotU heterodimerization during assembly of the Bacillus subtilis spore coat.
J Bacteriol: 2008, 190(4);1267-75
[PubMed:18065538]
[WorldCat.org]
[DOI]
(I p)
Rachele Isticato, Donatella Scotto Di Mase, Emilia M F Mauriello, Maurilio De Felice, Ezio Ricca
Amino terminal fusion of heterologous proteins to CotC increases display efficiencies in the Bacillus subtilis spore system.
Biotechniques: 2007, 42(2);151-2, 154, 156
[PubMed:17373477]
[WorldCat.org]
[DOI]
(P p)
Loredana Baccigalupi, Gaetano Castaldo, Giuseppina Cangiano, Rachele Isticato, Rosangela Marasco, Maurilio De Felice, Ezio Ricca
GerE-independent expression of cotH leads to CotC accumulation in the mother cell compartment during Bacillus subtilis sporulation.
Microbiology (Reading): 2004, 150(Pt 10);3441-9
[PubMed:15470121]
[WorldCat.org]
[DOI]
(P p)
Dinene L Crater, Kathryn H Wade, Orna Resnekov, H Travis Ichikawa, Lee Kroos, James A Brannigan, Charles P Moran
A mutation in GerE that affects cotC promoter activation in Bacillus subtilis.
Biochim Biophys Acta: 2002, 1576(1-2);30-8
[PubMed:12031481]
[WorldCat.org]
[DOI]
(P p)
H Ichikawa, L Kroos
Combined action of two transcription factors regulates genes encoding spore coat proteins of Bacillus subtilis.
J Biol Chem: 2000, 275(18);13849-55
[PubMed:10788508]
[WorldCat.org]
[DOI]
(P p)
L Zheng, R Halberg, S Roels, H Ichikawa, L Kroos, R Losick
Sporulation regulatory protein GerE from Bacillus subtilis binds to and can activate or repress transcription from promoters for mother-cell-specific genes.
J Mol Biol: 1992, 226(4);1037-50
[PubMed:1518043]
[WorldCat.org]
[DOI]
(P p)
L B Zheng, R Losick
Cascade regulation of spore coat gene expression in Bacillus subtilis.
J Mol Biol: 1990, 212(4);645-60
[PubMed:1691789]
[WorldCat.org]
[DOI]
(P p)
W Donovan, L B Zheng, K Sandman, R Losick
Genes encoding spore coat polypeptides from Bacillus subtilis.
J Mol Biol: 1987, 196(1);1-10
[PubMed:2821284]
[WorldCat.org]
[DOI]
(P p)
