NifS

• Description: cysteine desulfurase, required for NAD biosynthesis
  
  

Gene name	nifS
Synonyms	iscS
Essential	no
Product	cysteine desulfurase
Function	NAD biosynthesis
MW, pI	43 kDa, 6.425
Gene length, protein length	1185 bp, 395 aa
Immediate neighbours	nadB, nadR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU27880

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis of cofactors

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine (according to Swiss-Prot)

• Protein family: NifS/iscS subfamily (according to Swiss-Prot)

• Paralogous protein(s): YrvO, NifZ, Csd

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P38033

• KEGG entry: [3]

• E.C. number: 2.8.1.7

Expression and regulation

• Operon: nifS-nadR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of nicotinic acid (NadR) PubMed

• Regulatory mechanism:
  • NadR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: