MtlA

• Description: trigger enzyme: mannitol-specific phosphotransferase system, EIICB of the PTS
  
  

Gene name	mtlA
Synonyms
Essential	no
Product	trigger enzyme: mannitol-specific phosphotransferase system, EIICB
Function	mannitol uptake and phosphorylation, control of MtlR activity
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	65 kDa, 8.778
Gene length, protein length	1830 bp, 610 aa
Immediate neighbours	ycnL, mtlF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03981

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, trigger enzyme, membrane proteins, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

• Protein family: PTS permease, fructose/ mannitol permease (Fru) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-559 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: MtlA-MtlR PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1A3A (from Escherichia coli k12 mutant, 42% identity, 66% similarity) PubMed

• UniProt: P42956

• KEGG entry: [3]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: mtlA-mtlF-mtlD PubMed

• Sigma factor:

• Regulation:
  • induced by mannitol (MtlR) PubMed
  • repressed by glucose (MtlR) PubMed

• Regulatory mechanism:
  • MtlR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: