CtaB

• Description: heme O synthase (major enzyme)
  
  

Gene name	ctaB
Synonyms
Essential	no
Product	heme O synthase (major enzyme)
Function	heme biosynthesis
MW, pI	33 kDa, 9.566
Gene length, protein length	915 bp, 305 aa
Immediate neighbours	ctaA, ctaC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU14880

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis of cofactors, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ubiA prenyltransferase family, Protoheme IX farnesyltransferase subfamily (according to Swiss-Prot)

• Paralogous protein(s): CtaO

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P24009

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ctaB-ctaC-ctaD-ctaE-ctaF-ctaG PubMed

• Sigma factor:

• Regulation:
  • expressed under anaerobic conditions (ResD) PubMed

• Regulatory mechanism:
  • ResD: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tatsushi Mogi
Over-expression and characterization of Bacillus subtilis heme O synthase.
J Biochem: 2009, 145(5);669-75
[PubMed:19204012] [WorldCat.org] [DOI] (I p)

Brienne M Brown, Zhihong Wang, Kenneth R Brown, Julia A Cricco, Eric L Hegg
Heme O synthase and heme A synthase from Bacillus subtilis and Rhodobacter sphaeroides interact in Escherichia coli.
Biochemistry: 2004, 43(42);13541-8
[PubMed:15491161] [WorldCat.org] [DOI] (P p)

X Liu, H W Taber
Catabolite regulation of the Bacillus subtilis ctaBCDEF gene cluster.
J Bacteriol: 1998, 180(23);6154-63
[PubMed:9829923] [WorldCat.org] [DOI] (P p)

B Svensson, M Lübben, L Hederstedt
Bacillus subtilis CtaA and CtaB function in haem A biosynthesis.
Mol Microbiol: 1993, 10(1);193-201
[PubMed:7968515] [WorldCat.org] [DOI] (P p)