Difference between revisions of "ComEA"
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| − | + | * '''Description:''' exogenous DNA-binding protein <br/><br/> | |
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{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"|'''Function''' || competence | |style="background:#ABCDEF;" align="center"|'''Function''' || competence | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]''' |
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 4.824 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 4.824 | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[comEB]]'', ''[[comER]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[comEB]]'', ''[[comER]]'' | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+& | + | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14501]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' |
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| − | |colspan="2" | '''Genetic context''' | + | |colspan="2" | '''Genetic context''' <br/> [[Image:comEA_context.gif]] |
| − | + | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | |
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__TOC__ | __TOC__ | ||
| − | + | <br/><br/><br/><br/><br/><br/> | |
=The gene= | =The gene= | ||
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=References= | =References= | ||
| − | + | <pubmed>9987128,11814663,7768800,16751195,7968523,17630974,,19028902, </pubmed> | |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:37, 24 November 2010
- Description: exogenous DNA-binding protein
| Gene name | comEA |
| Synonyms | comD |
| Essential | no |
| Product | exogenous DNA-binding protein |
| Function | competence |
| Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation) | |
| MW, pI | 21 kDa, 4.824 |
| Gene length, protein length | 615 bp, 205 aa |
| Immediate neighbours | comEB, comER |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU25590
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 2DUY (from Thermus thermophilus hb8, 37% identity, 60% similarity)
- UniProt: P39694
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Mitsuo Ogura, Teruo Tanaka
The Bacillus subtilis late competence operon comE is transcriptionally regulated by yutB and under post-transcription initiation control by comN (yrzD).
J Bacteriol: 2009, 191(3);949-58
[PubMed:19028902]
[WorldCat.org]
[DOI]
(I p)
Naomi Kramer, Jeanette Hahn, David Dubnau
Multiple interactions among the competence proteins of Bacillus subtilis.
Mol Microbiol: 2007, 65(2);454-64
[PubMed:17630974]
[WorldCat.org]
[DOI]
(P p)
Inês Chen, Roberta Provvedi, David Dubnau
A macromolecular complex formed by a pilin-like protein in competent Bacillus subtilis.
J Biol Chem: 2006, 281(31);21720-21727
[PubMed:16751195]
[WorldCat.org]
[DOI]
(P p)
Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol Lett: 2002, 206(2);197-200
[PubMed:11814663]
[WorldCat.org]
[DOI]
(P p)
R Provvedi, D Dubnau
ComEA is a DNA receptor for transformation of competent Bacillus subtilis.
Mol Microbiol: 1999, 31(1);271-80
[PubMed:9987128]
[WorldCat.org]
[DOI]
(P p)
G S Inamine, D Dubnau
ComEA, a Bacillus subtilis integral membrane protein required for genetic transformation, is needed for both DNA binding and transport.
J Bacteriol: 1995, 177(11);3045-51
[PubMed:7768800]
[WorldCat.org]
[DOI]
(P p)
J Hahn, G Inamine, Y Kozlov, D Dubnau
Characterization of comE, a late competence operon of Bacillus subtilis required for the binding and uptake of transforming DNA.
Mol Microbiol: 1993, 10(1);99-111
[PubMed:7968523]
[WorldCat.org]
[DOI]
(P p)
