Difference between revisions of "CshB"
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| − | * '''Description:''' DEAD-box RNA helicase<br/><br/> | + | * '''Description:''' [[DEAD-box RNA helicases|DEAD-box RNA helicase]]<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || DEAD-box RNA helicase | + | |style="background:#ABCDEF;" align="center"| '''Product''' || [[DEAD-box RNA helicases|DEAD-box RNA helicase]] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || RNA helicase | |style="background:#ABCDEF;" align="center"|'''Function''' || RNA helicase | ||
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* '''Catalyzed reaction/ biological activity:''' RNA helicase | * '''Catalyzed reaction/ biological activity:''' RNA helicase | ||
| − | * '''Protein family:''' DEAD box | + | * '''Protein family:''' [[DEAD-box RNA helicases|DEAD-box RNA helicase]] |
* '''Paralogous protein(s):''' [[CshA]], [[DeaD]], [[YfmL]] | * '''Paralogous protein(s):''' [[CshA]], [[DeaD]], [[YfmL]] | ||
Revision as of 15:17, 20 November 2010
- Description: DEAD-box RNA helicase
| Gene name | cshB |
| Synonyms | yqfR |
| Essential | no |
| Product | DEAD-box RNA helicase |
| Function | RNA helicase |
| MW, pI | 49 kDa, 9.89 |
| Gene length, protein length | 1314 bp, 438 aa |
| Immediate neighbours | nfo, yqfQ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU25140
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: RNA helicase
- Protein family: DEAD-box RNA helicase
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasma, colocalizes with the ribosomes PubMed
Database entries
- Structure:
- UniProt: P54475
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1002 (erm), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Mohamed Marahiel, Marburg University, Germany homepage
Your additional remarks
References
Martin Lehnik-Habrink, Henrike Pförtner, Leonie Rempeters, Nico Pietack, Christina Herzberg, Jörg Stülke
The RNA degradosome in Bacillus subtilis: identification of CshA as the major RNA helicase in the multiprotein complex.
Mol Microbiol: 2010, 77(4);958-71
[PubMed:20572937]
[WorldCat.org]
[DOI]
(I p)
Karen Hunger, Carsten L Beckering, Frank Wiegeshoff, Peter L Graumann, Mohamed A Marahiel
Cold-induced putative DEAD box RNA helicases CshA and CshB are essential for cold adaptation and interact with cold shock protein B in Bacillus subtilis.
J Bacteriol: 2006, 188(1);240-8
[PubMed:16352840]
[WorldCat.org]
[DOI]
(P p)
Carsten L Beckering, Leif Steil, Michael H W Weber, Uwe Völker, Mohamed A Marahiel
Genomewide transcriptional analysis of the cold shock response in Bacillus subtilis.
J Bacteriol: 2002, 184(22);6395-402
[PubMed:12399512]
[WorldCat.org]
[DOI]
(P p)
Additional publications: PubMed
