GapA

• Synonyms:

• Description: glyceraldehyde-3-phosphate dehydrogenase, (NADH-dependent), glycolytic enzyme

The gene

Basic information

• Coordinates:

• Gene length:

Phenotypes of a mutant

essential

Database entries

• DBTBS entry: [1]

• SubtiList entry:[2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: glyceraldehyde-3-phosphate dehydrogenase, (NADH-dependent)

• Protein family:

• Paralogous protein(s): GapB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: Phosphorylation (STY)

• Effectors of protein activity:

• Interactions:
  • GapA-PtsH: HPr(Ser-46-P) binds GapA resulting in a slight inhibition of enzymatic activity.
  • GapA-Crh: Crh(Ser-46-P) binds GapA resulting in a slight inhibition of enzymatic activity.

• Localization: membrane and cytoplasm

Database entries

• Structure:
  • 3CMC (from Geobacillus stearothermophilus)
  • 1NQO (from Geobacillus stearothermophilus, mutant with cys 149 replaced by ser, complex with NAD+ und D-Glyceraldehyde-3-Phosphate)

• Swiss prot entry:

• KEGG entry:

Expression and regulation

• Operon: cggR-gapA-pgk-tpiA-pgm-eno
• Sigma factor: SigA

• Regulation: CggR represses the operon in the absence of glycolytic sugars

• Regulatory mechanism: repression

• Database entries: DBTBS

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• Antibody:

References

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. [3]