Difference between revisions of "LcfA"

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* '''Description:''' long chain acyl-CoA synthetase <br/><br/>
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* '''Description:''' long chain acyl-CoA synthetase, involved in surfactin production <br/><br/>
  
 
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{| align="right" border="1" cellpadding="2"  
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot)  
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* '''Catalyzed reaction/ biological activity:''' ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis {{PubMed|20797616}}
  
 
* '''Protein family:''' ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
 
* '''Protein family:''' ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
  
* '''Paralogous protein(s):'''
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* '''Paralogous protein(s):''' [[LcfB]], [[YhfT]], [[YngI]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
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* '''Mutant:''' available in [[Mohamed Marahiel]]'s lab {{PubMed|20797616}}
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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=References=
 
=References=
  
<pubmed>17189250,17919287</pubmed>
+
<pubmed>17189250,17919287 20797616</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:21, 1 September 2010

  • Description: long chain acyl-CoA synthetase, involved in surfactin production

Gene name lcfA
Synonyms
Essential no
Product long chain acyl-CoA synthetase
Function fatty acid degradation
Metabolic function and regulation of this protein in SubtiPathways:
Fatty acid degradation
MW, pI 62 kDa, 6.119
Gene length, protein length 1680 bp, 560 aa
Immediate neighbours fadR, yshE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LcfA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU28560

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed
  • Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by glucose (CcpA)
    • induced by long chain acyl-CoA (C14 ... C20) (FadR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References