Difference between revisions of "Tkt"

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(Extended information on the protein)
(References)
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[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 19:25, 31 July 2010

  • Description: transketolase

Gene name tkt
Synonyms tktA
Essential no
Product transketolase
Function pentose phosphate pathway
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 72 kDa, 4.803
Gene length, protein length 2001 bp, 667 aa
Immediate neighbours ynzC, sirA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Tkt context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU17890

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate (according to Swiss-Prot)
  • Protein family: transketolase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on ser/ thr/ tyr PubMed
  • Cofactor(s): thiamine pyrophosphate
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: [3K95], complex with thiamine diphosphate, from B. anthracis
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: BS4530 (aphA3), available in Stülke lab
  • Expression vector:
    • pGP94 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Stülke lab
    • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP820, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References