Difference between revisions of "CshB"

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(Biological materials)
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:''' [[CshB]]-[[CspB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16352840 PubMed]
+
* '''Interactions:''' [[CshB]]-[[CspB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16352840 PubMed], [[CshB]]-[[DeaD]] {{PubMed|20572937}}
  
 
* '''Localization:''' cytoplasma, colocalizes with the ribosomes [http://www.ncbi.nlm.nih.gov/sites/entrez/16352840 PubMed]
 
* '''Localization:''' cytoplasma, colocalizes with the ribosomes [http://www.ncbi.nlm.nih.gov/sites/entrez/16352840 PubMed]
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* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** well expressed in complex medium, poorly expressed in minimal medium {{PubMed|20572937}}
 +
** expression declines in stationary phase {{PubMed|20572937}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
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=References=
 
=References=
  
<pubmed>16352840,, 12399512, </pubmed>
+
<pubmed>16352840,, 12399512, 20572937</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:27, 25 June 2010

  • Description: DEAD-box RNA helicase

Gene name cshB
Synonyms yqfR
Essential no
Product DEAD-box RNA helicase
Function RNA helicase
MW, pI 49 kDa, 9.89
Gene length, protein length 1314 bp, 438 aa
Immediate neighbours nfo, yqfQ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YqfR context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU25140

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: RNA helicase
  • Protein family: DEAD box helicase family (according to Swiss-Prot) DEAD-box RNA helicase

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cytoplasma, colocalizes with the ribosomes PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • well expressed in complex medium, poorly expressed in minimal medium PubMed
    • expression declines in stationary phase PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1002 (erm), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • FLAG-tag construct: GP1011 (spc, based on pGP1331), available in the Stülke labs
  • Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

Your additional remarks

References