Difference between revisions of "MmgD"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' [[mmgA]]-[[mmgB]]-[[mmgC]]-[[mmgD]]-[[mmgE]]-[[yqiQ]] {{PubMed|8759838}}
+
* '''Operon:''' ''[[mmgA]]-[[mmgB]]-[[mmgC]]-[[mmgD]]-[[mmgE]]-[[yqiQ]]'' {{PubMed|8759838}}
  
 
* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|15699190,8759838}}
 
* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|15699190,8759838}}
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**[[CcpA]]: transcription repression {{PubMed|8759838}}
 
**[[CcpA]]: transcription repression {{PubMed|8759838}}
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 20:47, 12 June 2010

  • Description: 2-methylcitrate synthase

Gene name mmgD
Synonyms yqiO, prpC
Essential no
Product 2-methylcitrate synthase
Function mother cell metabolism
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 41 kDa, 6.202
Gene length, protein length 1116 bp, 372 aa
Immediate neighbours mmgE, mmgC
Gene sequence (+200bp) Protein sequence
Genetic context
MmgD context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU24140

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Propanoyl-CoA + H2O + oxaloacetate = (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA (according to Swiss-Prot)
  • Protein family: citrate synthase family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed early during sporulation in the mother cell (SigE) PubMed
    • repressed by glucose (CcpA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

E M Bryan, B W Beall, C P Moran
A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis.
J Bacteriol: 1996, 178(16);4778-86
[PubMed:8759838] [WorldCat.org] [DOI] (P p)