YfnI

• Description: general stress protein, minor lipoteichoic acid synthetase, major secreted protein
  
  

Gene name	yfnI
Synonyms	yetP, ltaSA
Essential	no
Product	minor lipoteichoic acid synthetase
Function	biosynthesis of lipoteichoic acid
MW, pI	73 kDa, 5.951
Gene length, protein length	1917 bp, 639 aa
Immediate neighbours	yetO, yfnH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU07260

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LTA synthase family (according to Swiss-Prot)

• Paralogous protein(s): LtaS, YqgS, YvgJ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on (Thr-311 OR Ser-312) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: Q797B3

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yfnI (according to DBTBS)

• Sigma factor: SigB PubMed, SigM PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • SM-NB (yfnI-spc), available in Anne Galinier's and Boris Görke's labs

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kathrin Schirner, Jon Marles-Wright, Richard J Lewis, Jeff Errington
Distinct and essential morphogenic functions for wall- and lipo-teichoic acids in Bacillus subtilis.
EMBO J: 2009, 28(7);830-42
[PubMed:19229300] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Adrian J Jervis, Penny D Thackray, Chris W Houston, Malcolm J Horsburgh, Anne Moir
SigM-responsive genes of Bacillus subtilis and their promoters.
J Bacteriol: 2007, 189(12);4534-8
[PubMed:17434969] [WorldCat.org] [DOI] (P p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)