PtsH

• Description: HPr, General component of the sugar phosphotransferase system (PTS).
  
  

Gene name	ptsH
Synonyms
Essential	no
Product	histidine-containing phosphocarrier protein HPr of the PTS
Function	PTS-dependent sugar transport and carbon catabolite repression
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism
MW, pI	9,1 kDa, 4.58
Gene length, protein length	264 bp, 88 amino acids
Immediate neighbours	ptsG, ptsI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU13900

Database entries

• DBTBS entry: [1]

• SubtiList entry:[2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine (according to Swiss-Prot) Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine

• Protein family: HPr domain (according to Swiss-Prot) HPr family

• Paralogous protein(s): Crh

Extended information on the protein

• Kinetic information:

• Domains: HPr Domain (2–88)

• Modification:
  • transient phosphorylation by Enzyme I of the PTS on His-15
  • regulatory phosphorylation on Ser-46 by HprK PubMed
  • weak phosphorylation on Ser-12 PubMed
  • an extensive study on in vivo HPr phosphorylation can be found in Singh et al. (2008) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: HPr-LicT, HPr-SacY, HPr-SacT, HPr-GlcT, HPr-GlpK, GapA-HPr PubMed, HPr-MtlR, HPr-LicR, HPr-LevR,HPr-ManR, HPr(Ser-46)-P-CcpA PubMed, HPr-RbsR PubMed, HprK-HPr PubMed, HPr(His)-P-YesS PubMed, HPr-PtsG PubMed

• Localization: cytoplasm (according to Swiss-Prot), Cytoplasm PubMed

Database entries

• Structure: 1KKM (complex of L. casei HprK with B. subtilis HPr-Ser-P), 1KKL (complex of Lactobacillus casei HprK with B. subtilis HPr), 2HID (NMR)

• UniProt: P08877

• KEGG entry: [3]

• E.C. number: 2.7.11.-

Expression and regulation

• Operon:
  • ptsG-ptsH-ptsI PubMed
  • ptsH-ptsI PubMed

• Sigma factor:
  • ptsG: SigA PubMed
  • ptsH: SigA PubMed

• Regulation:
  • expression activated by glucose (2 fold) (GlcT) PubMed
  • the ptsH promoter is constitutive PubMed
  • subject to negative stringent control upon amino acid limitation (due to control of ptsG transcription initiation) PubMed

• Regulatory mechanism:
  • ptsG: transcriptional antitermination via the GlcT-dependent RNA switch PubMed

• Additional information:

Biological materials

• Mutant: MZ303 (cat), GP507 ptsH1 (S46A), GP506 (ptsH-H15A), available in Stülke lab

• Expression vector:
  • pGP438 (with N-terminal Strep-tag, in pGP172), available in Stülke lab
  • pAG2 (His-tag), available in Anne Galinier lab
  • pGP371(expression / purification of HPr-S46A, with His-tag from E. coli, in pWH844), available in Stülke lab
  • pGP1415 (HPr, expression in B. subtilis, from pBQ200), available in Stülke lab
  • pGP1416 (HPr-H15A, expression in B. subtilis, from pBQ200), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

• Antibody: available in Stülke lab

Labs working on this gene/protein

Josef Deutscher, Paris-Grignon, France

Jörg Stülke, University of Göttingen, Germany Homepage

Wolfgang Hillen, Erlangen University, Germany Homepage

Richard Brennan, Houston, Texas, USA Homepage

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France