Difference between revisions of "FliG"
(→Expression and regulation) |
|||
| Line 68: | Line 68: | ||
* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
| − | * '''Effectors of protein activity:''' | + | * '''Effectors of protein activity:''' interaction with [[EpsE]] prevents energetization of the flagellum by [[MotA]]-[[MotB]] {{PubMed|18566286}} |
| − | * '''Interactions:''' | + | * '''Interactions:''' [[EpsE]]-[[FliG]] {{PubMed|18566286}} |
* '''Localization:''' cell membrane (according to Swiss-Prot) | * '''Localization:''' cell membrane (according to Swiss-Prot) | ||
| Line 120: | Line 120: | ||
=References= | =References= | ||
| − | <pubmed>1905667,9657996,8157612,15175317,14651647, </pubmed> | + | <pubmed>1905667,9657996,8157612,15175317,14651647, 18566286 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:13, 28 March 2010
- Description: flagellar motor switch protein
| Gene name | fliG |
| Synonyms | flaA15 |
| Essential | no |
| Product | flagellar motor switch protein |
| Function | movement and chemotaxis |
| MW, pI | 38 kDa, 4.46 |
| Gene length, protein length | 1014 bp, 338 aa |
| Immediate neighbours | fliF, fliH |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU16220
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: fliG family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity: interaction with EpsE prevents energetization of the flagellum by MotA-MotB PubMed
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P23448
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-ylxG-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-ylxH-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Kris M Blair, Linda Turner, Jared T Winkelman, Howard C Berg, Daniel B Kearns
A molecular clutch disables flagella in the Bacillus subtilis biofilm.
Science: 2008, 320(5883);1636-8
[PubMed:18566286]
[WorldCat.org]
[DOI]
(I p)
H Werhane, P Lopez, M Mendel, M Zimmer, G W Ordal, L M Márquez-Magaña
The last gene of the fla/che operon in Bacillus subtilis, ylxL, is required for maximal sigmaD function.
J Bacteriol: 2004, 186(12);4025-9
[PubMed:15175317]
[WorldCat.org]
[DOI]
(P p)
Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647]
[WorldCat.org]
[DOI]
(P p)
W Estacio, S S Anna-Arriola, M Adedipe, L M Márquez-Magaña
Dual promoters are responsible for transcription initiation of the fla/che operon in Bacillus subtilis.
J Bacteriol: 1998, 180(14);3548-55
[PubMed:9657996]
[WorldCat.org]
[DOI]
(P p)
L M Márquez-Magaña, M J Chamberlin
Characterization of the sigD transcription unit of Bacillus subtilis.
J Bacteriol: 1994, 176(8);2427-34
[PubMed:8157612]
[WorldCat.org]
[DOI]
(P p)
A R Zuberi, C Ying, D S Bischoff, G W Ordal
Gene-protein relationships in the flagellar hook-basal body complex of Bacillus subtilis: sequences of the flgB, flgC, flgG, fliE and fliF genes.
Gene: 1991, 101(1);23-31
[PubMed:1905667]
[WorldCat.org]
[DOI]
(P p)
