Difference between revisions of "SpoIID"

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* '''Description:''' required for complete dissolution of the asymmetric septum <br/><br/>
+
* '''Description:''' cell wall hydrolase (lytic transglycosylase), required for complete dissolution of the asymmetric septum <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || lytic transglycosylase
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || sporulation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || sporulation
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:'''  
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* '''Catalyzed reaction/ biological activity:''' degrades the glycan strands of the peptidoglycan into disaccharide units {{PubMed|20159959}}, enhances [[SpoIIP]] activity {{PubMed|20159959}}
  
 
* '''Protein family:'''
 
* '''Protein family:'''
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:'''
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* '''Interactions:''' [[SpoIID]]-[[SpoIIP]] {{PubMed|20159959}}
  
* '''Localization:'''
+
* '''Localization:''' cell membrane {{PubMed|20159959}}
  
 
=== Database entries ===
 
=== Database entries ===
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=References=
 
=References=
  
<pubmed>17376078,1946462,11886548,15752199 3011962 15699190, 15383836 </pubmed>
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<pubmed>17376078,1946462,11886548,15752199 3011962 15699190, 15383836 20159959 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:07, 18 February 2010

  • Description: cell wall hydrolase (lytic transglycosylase), required for complete dissolution of the asymmetric septum

Gene name spoIID
Synonyms spoIIC
Essential no
Product lytic transglycosylase
Function sporulation
MW, pI 37 kDa, 9.764
Gene length, protein length 1029 bp, 343 aa
Immediate neighbours ywmC, murAA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SpoIID context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU36750

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: degrades the glycan strands of the peptidoglycan into disaccharide units PubMed, enhances SpoIIP activity PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Cecile Morlot, Tsuyoshi Uehara, Kathleen A Marquis, Thomas G Bernhardt, David Z Rudner
A highly coordinated cell wall degradation machine governs spore morphogenesis in Bacillus subtilis.
Genes Dev: 2010, 24(4);411-22
[PubMed:20159959] [WorldCat.org] [DOI] (I p)

Arnaud Chastanet, Richard Losick
Engulfment during sporulation in Bacillus subtilis is governed by a multi-protein complex containing tandemly acting autolysins.
Mol Microbiol: 2007, 64(1);139-52
[PubMed:17376078] [WorldCat.org] [DOI] (P p)

Thierry Doan, Kathleen A Marquis, David Z Rudner
Subcellular localization of a sporulation membrane protein is achieved through a network of interactions along and across the septum.
Mol Microbiol: 2005, 55(6);1767-81
[PubMed:15752199] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

P Eichenberger, P Fawcett, R Losick
A three-protein inhibitor of polar septation during sporulation in Bacillus subtilis.
Mol Microbiol: 2001, 42(5);1147-62
[PubMed:11886548] [WorldCat.org] [DOI] (P p)

A Driks, R Losick
Compartmentalized expression of a gene under the control of sporulation transcription factor sigma E in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1991, 88(22);9934-8
[PubMed:1946462] [WorldCat.org] [DOI] (P p)

I Lopez-Diaz, S Clarke, J Mandelstam
spoIID operon of Bacillus subtilis: cloning and sequence.
J Gen Microbiol: 1986, 132(2);341-54
[PubMed:3011962] [WorldCat.org] [DOI] (P p)