NupC

• Description: pyrimidine nucleoside transport protein
  
  

Gene name	nupC
Synonyms
Essential	no
Product	pyrimidine nucleoside transport protein
Function	pyrimidine uptake
Metabolic function and regulation of this protein in SubtiPathways: Nucleoside catabolism, Nucleotides (regulation)
MW, pI	42 kDa, 9.316
Gene length, protein length	1179 bp, 393 aa
Immediate neighbours	pdp, dra
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39410

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: SLC28A transporter family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot), membrane associated PubMed

Database entries

• Structure:

• UniProt: P39141

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dra-nupC-pdp PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by deoxynucleotides (DeoR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • DeoR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Xianmin Zeng, Anne Galinier, Hans H Saxild
Catabolite repression of dra-nupC-pdp operon expression in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 11);2901-2908
[PubMed:11065368] [WorldCat.org] [DOI] (P p)

Y Miwa, A Nakata, A Ogiwara, M Yamamoto, Y Fujita
Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis.
Nucleic Acids Res: 2000, 28(5);1206-10
[PubMed:10666464] [WorldCat.org] [DOI] (I p)

X Zeng, H H Saxild
Identification and characterization of a DeoR-specific operator sequence essential for induction of dra-nupC-pdp operon expression in Bacillus subtilis.
J Bacteriol: 1999, 181(6);1719-27
[PubMed:10074062] [WorldCat.org] [DOI] (P p)

H H Saxild, L N Andersen, K Hammer
Dra-nupC-pdp operon of Bacillus subtilis: nucleotide sequence, induction by deoxyribonucleosides, and transcriptional regulation by the deoR-encoded DeoR repressor protein.
J Bacteriol: 1996, 178(2);424-34
[PubMed:8550462] [WorldCat.org] [DOI] (P p)