Difference between revisions of "RocB"

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=Expression and regulation=
 
=Expression and regulation=
 
 
* '''Operon:''' ''[[rocA]]-[[rocB]]-[[rocC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162 PubMed]
 
* '''Operon:''' ''[[rocA]]-[[rocB]]-[[rocC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162 PubMed]
  
 
* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162 PubMed]
 
* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162 PubMed]
  
* '''Regulation:''' induction by arginine ([[RocR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162 PubMed], repressed by [[CodY]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
+
* '''Regulation:'''  
 +
** induction by arginine ([[RocR]], [[AhrC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162,7565595 PubMed]
 
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
 
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
  
* '''Regulatory mechanism:''' [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed], [[RocR]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162 PubMed]
+
* '''Regulatory mechanism:'''  
 
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
 
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
 +
** [[RocR]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162 PubMed]
 +
** [[AhrC]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/7565595 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
  
<pubmed>12618455,,12618455,8113162, </pubmed>
+
<pubmed>7565595 12618455,8113162, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 20:35, 3 February 2010

  • Description: involved in arginine and ornithine utilization

Gene name rocB
Synonyms ipa-77d
Essential no
Product unknown
Function arginine, ornithine and citrulline utilization
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 64 kDa, 5.347
Gene length, protein length 1698 bp, 566 aa
Immediate neighbours rocC, rocA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RocB context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU37770

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): YqjN

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induction by arginine (RocR, AhrC) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

U Klingel, C M Miller, A K North, P G Stockley, S Baumberg
A binding site for activation by the Bacillus subtilis AhrC protein, a repressor/activator of arginine metabolism.
Mol Gen Genet: 1995, 248(3);329-40
[PubMed:7565595] [WorldCat.org] [DOI] (P p)

S Calogero, R Gardan, P Glaser, J Schweizer, G Rapoport, M Debarbouille
RocR, a novel regulatory protein controlling arginine utilization in Bacillus subtilis, belongs to the NtrC/NifA family of transcriptional activators.
J Bacteriol: 1994, 176(5);1234-41
[PubMed:8113162] [WorldCat.org] [DOI] (P p)