Difference between revisions of "IolE"

From SubtiWiki
Jump to: navigation, search
(Expression and regulation)
Line 95: Line 95:
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** repressed in the absence of inositol ([[IolR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9887260 PubMed]
 
 
** repressed by glucose ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10666464 PubMed]  
 
** repressed by glucose ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10666464 PubMed]  
** induced by inositol ([[IolR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed]
+
** induced by inositol ([[IolR]]) {{PubMed|9887260,9226270}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** [[IolR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9887260 PubMed]
+
** [[IolR]]: transcription repression {{PubMed|9887260,9226270}}
 
** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10666464 PubMed]
 
** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10666464 PubMed]
** [[IolR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed]
 
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 22:08, 11 December 2009

  • Description: 2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)

Gene name iolE
Synonyms yxdE
Essential no
Product 2-keto-myo-inositol dehydratase,
dehydration of 2-keto-myo-inositol (2nd reaction)
Function myo-inositol catabolism
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 33 kDa, 5.209
Gene length, protein length 891 bp, 297 aa
Immediate neighbours iolF, iolD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
IolE context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU39720

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2,4,6/3,5-pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H2O (according to Swiss-Prot)
  • Protein family: iolE/mocC family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ken-ichi Yoshida, Masanori Yamaguchi, Tetsuro Morinaga, Masaki Kinehara, Maya Ikeuchi, Hitoshi Ashida, Yasutaro Fujita
myo-Inositol catabolism in Bacillus subtilis.
J Biol Chem: 2008, 283(16);10415-24
[PubMed:18310071] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Masanori Yamaguchi, Hideki Ikeda, Kaoru Omae, Ken-Ichi Tsurusaki, Yasutaro Fujita
The fifth gene of the iol operon of Bacillus subtilis, iolE, encodes 2-keto-myo-inositol dehydratase.
Microbiology (Reading): 2004, 150(Pt 3);571-580
[PubMed:14993306] [WorldCat.org] [DOI] (P p)

K I Yoshida, T Shibayama, D Aoyama, Y Fujita
Interaction of a repressor and its binding sites for regulation of the Bacillus subtilis iol divergon.
J Mol Biol: 1999, 285(3);917-29
[PubMed:9887260] [WorldCat.org] [DOI] (P p)

K I Yoshida, D Aoyama, I Ishio, T Shibayama, Y Fujita
Organization and transcription of the myo-inositol operon, iol, of Bacillus subtilis.
J Bacteriol: 1997, 179(14);4591-8
[PubMed:9226270] [WorldCat.org] [DOI] (P p)