Difference between revisions of "GntR"

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Line 80: Line 80:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P10585 P10585]
+
* '''UniProt:''' [http://www.uniprot.org/uniprot/P10585 P10585]
  
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU40050]
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU40050]

Revision as of 15:25, 20 July 2009

  • Description: transcriptional repressor of the gluconate operon

Gene name gntR
Synonyms
Essential no
Product transcriptional repressor of the gluconate operon
Function regulation of gluconate utilization
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 28 kDa, 5.26
Gene length, protein length 729 bp, 243 aa
Immediate neighbours yxaA, gntK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GntR context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU40050

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: gluconate acts as molecular inducer
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)

Y Miwa, K Nagura, S Eguchi, H Fukuda, J Deutscher, Y Fujita
Catabolite repression of the Bacillus subtilis gnt operon exerted by two catabolite-responsive elements.
Mol Microbiol: 1997, 23(6);1203-13
[PubMed:9106211] [WorldCat.org] [DOI] (P p)

J Reizer, U Bergstedt, A Galinier, E Küster, M H Saier, W Hillen, M Steinmetz, J Deutscher
Catabolite repression resistance of gnt operon expression in Bacillus subtilis conferred by mutation of His-15, the site of phosphoenolpyruvate-dependent phosphorylation of the phosphocarrier protein HPr.
J Bacteriol: 1996, 178(18);5480-6
[PubMed:8808939] [WorldCat.org] [DOI] (P p)

K Yoshida, Y Miwa, H Ohmori, Y Fujita
Analysis of an insertional operator mutation (gntOi) that affects the expression level of the Bacillus subtilis gnt operon, and characterization of gntOi suppressor mutations.
Mol Gen Genet: 1995, 248(5);583-91
[PubMed:7476858] [WorldCat.org] [DOI] (P p)

Y Fujita, Y Miwa, A Galinier, J Deutscher
Specific recognition of the Bacillus subtilis gnt cis-acting catabolite-responsive element by a protein complex formed between CcpA and seryl-phosphorylated HPr.
Mol Microbiol: 1995, 17(5);953-60
[PubMed:8596444] [WorldCat.org] [DOI] (P p)

K Yoshida, H Ohmori, Y Miwa, Y Fujita
Bacillus subtilis gnt repressor mutants that diminish gluconate-binding ability.
J Bacteriol: 1995, 177(16);4813-6
[PubMed:7642511] [WorldCat.org] [DOI] (P p)

Y Fujita, Y Miwa
Catabolite repression of the Bacillus subtilis gnt operon mediated by the CcpA protein.
J Bacteriol: 1994, 176(2);511-3
[PubMed:8288545] [WorldCat.org] [DOI] (P p)

Y Miwa, Y Fujita
Promoter-independent catabolite repression of the Bacillus subtilis gnt operon.
J Biochem: 1993, 113(6);665-71
[PubMed:8370661] [WorldCat.org] [DOI] (P p)

K Yoshida, Y Fujita, A Sarai
Missense mutations in the Bacillus subtilis gnt repressor that diminish operator binding ability.
J Mol Biol: 1993, 231(2);167-74
[PubMed:8510140] [WorldCat.org] [DOI] (P p)

A Reizer, J Deutscher, M H Saier, J Reizer
Analysis of the gluconate (gnt) operon of Bacillus subtilis.
Mol Microbiol: 1991, 5(5);1081-9
[PubMed:1659648] [WorldCat.org] [DOI] (P p)

Y Miwa, Y Fujita
Determination of the cis sequence involved in catabolite repression of the Bacillus subtilis gnt operon; implication of a consensus sequence in catabolite repression in the genus Bacillus.
Nucleic Acids Res: 1990, 18(23);7049-53
[PubMed:2124676] [WorldCat.org] [DOI] (P p)

Y Fujita, T Fujita, Y Miwa
Evidence for posttranscriptional regulation of synthesis of the Bacillus subtilis Gnt repressor.
FEBS Lett: 1990, 267(1);71-4
[PubMed:2163901] [WorldCat.org] [DOI] (P p)

Y Fujita, Y Miwa
Identification of an operator sequence for the Bacillus subtilis gnt operon.
J Biol Chem: 1989, 264(7);4201-6
[PubMed:2492998] [WorldCat.org] (P p)

Y Miwa, Y Fujita
Purification and characterization of a repressor for the Bacillus subtilis gnt operon.
J Biol Chem: 1988, 263(26);13252-7
[PubMed:2843515] [WorldCat.org] (P p)

Y Fujita, T Fujita
The gluconate operon gnt of Bacillus subtilis encodes its own transcriptional negative regulator.
Proc Natl Acad Sci U S A: 1987, 84(13);4524-8
[PubMed:3037520] [WorldCat.org] [DOI] (P p)

Y Fujita, T Fujita, Y Miwa, J Nihashi, Y Aratani
Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis.
J Biol Chem: 1986, 261(29);13744-53
[PubMed:3020045] [WorldCat.org] (P p)

Y Fujita, T Fujita
Identification and nucleotide sequence of the promoter region of the Bacillus subtilis gluconate operon.
Nucleic Acids Res: 1986, 14(3);1237-52
[PubMed:2419835] [WorldCat.org] [DOI] (P p)

Y Fujita, J Nihashi, T Fujita
The characterization and cloning of a gluconate (gnt) operon of Bacillus subtilis.
J Gen Microbiol: 1986, 132(1);161-9
[PubMed:3011959] [WorldCat.org] [DOI] (P p)